problem in PCR clonong

Karl Fischer tyr-2 at bones.biochem.ualberta.ca
Thu Jul 31 15:12:54 EST 1997


In article <5rqf9v$c88 at sifon.cc.mcgill.ca>, czlq at musica.mcgill.ca wrote:

>The PCR product I used are either the original PCR product or PCR
product    purified  by ethanol precipitation.

Lee,

Make sure you get rid of unused oligo's from the reaction by gel
purification of your fragment or using a commercial kit to clean your
product (eg. Promega PCR clean-up); ethanol precipitation can still result
in respectable levels of oligo precipitating with your fragment. You'll
find that by rendering your fragment oligo free the number of false
positives will drop significantly.

As usual - no affiliations with Promega.

Cheers

Karl the hepB guy

-- 
Karl Fischer
tyr-2 at bones.biochem.ualberta.ca





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