Plating Density for colonies in E.coli Amplification?

Franck Laurent franck.laurent at medew.fyto.wau.nl
Wed Jul 30 13:16:31 EST 1997


In article <5rmtld$k58 at nntp3.u.washington.edu>, polliwog at u.washington.edu 
says...
>
>
>I want to amplify a cDNA library on 150mm petri dishes.
>What is a good number of cfu to aim for per plate?
>
>Thanks
>SDF
>-- 
>Seth D. Findley   
>Department of Biochemistry
>Health Sciences Building J579
>University of Washington, Box 357350, Seattle, WA 98195


40,000 cfu/plate should be fine.
Franck




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