Plating Density for colonies in E.coli Amplification?
franck.laurent at medew.fyto.wau.nl
Wed Jul 30 13:16:31 EST 1997
In article <5rmtld$k58 at nntp3.u.washington.edu>, polliwog at u.washington.edu
>I want to amplify a cDNA library on 150mm petri dishes.
>What is a good number of cfu to aim for per plate?
>Seth D. Findley
>Department of Biochemistry
>Health Sciences Building J579
>University of Washington, Box 357350, Seattle, WA 98195
40,000 cfu/plate should be fine.
More information about the Methods