PCR product stuck in well

Tom tom at senet.com.au
Wed Jul 30 17:45:10 EST 1997


Hi,

Am doing a 4kb PCR off a 7kb plasmid, but when run on a 0.8% agarose
gel, there seems to be a heap of DNA stuck in the well, and the rest
of the lane blank. PhOH/Chloroform nor PCR wizard preps help, nor does
diluting the product before gelling. Nor is it template.

Any ideas??? I presume I haven't performed a >100kb PCR!! 

Cheers,
tom

tom at senet.com.au




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