Prove one cDNA consists of 2 clones

Zhonglin Chai zchai at COBRA.PATH.MONASH.EDU.AU
Thu Jul 31 20:23:55 EST 1997

You may do a RT-PCR using primers outside the overlapping regions from each
clone. If you are derived from a single mRNA you should be able to get a
fragment amplified.
hope this of help.

>     We have picked up 2 clones from a cDNA library which show they have
>500nt overlap area (most probably completely identical, we will know
>soon). I wonder if someone can tell me the best way(methods) to prove
>they are one transcript directly from mRNA level. Thanks!
>     Cheng

ZhongLin Chai, PhD
Department of Pathology and Immunology
Monash University Medical School
Alfred Hospital, Commercial Rd,
Prahran, Victoria, AUSTRALIA 3181
Telephone: (61 3) 9276 2698 (lab)
           (61 3) 9276 2696 (office)
           (61 3) 9384 1305 (home)
           041 987 1940 (mobile within Australia)
           +61 419 87 1940 (mobile international)
Fax:       (61 3) 9529 6484
email:     zchai at

More information about the Methods mailing list