Vent Polymerase

A.Al-dukhyil MBQ96AA at sheffield.ac.uk
Sun Jun 1 03:07:17 EST 1997


Dear  Dr. ZhongLin Chai
Many thanks for your quik answer.
Best regards.

> Date:          Sun, 1 Jun 1997 12:22:44 +1000
> To:            A Al-dukhyil <MBQ96AA at shef.ac.uk>
> From:          zchai at cobra.path.monash.edu.au (Zhonglin Chai)
> Subject:       Re: Vent Polymerase
> Cc:            methods at net.bio.net

> Yes, you do. I think Vent and other enzymes with proof reading activities
> have both 5' -> 3' polymerization activity and 3' -> 5' exonuclease
> activity. As soon as the 3' -> 5' exonuclease activity delete the nt from
> the 3' end and leave the opposite DNA strand with a 5' overhang, the 5' ->
> 3' polymerization activity would add nt to the 3' end according to the
> opposite strand sequence, that needs dNTPs as building materials.
> 
> 
> 
> Dr. ZhongLin Chai
> ____________________________________________
> Department of Pathology and Immunology
> Monash University Medical School
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