which enzymes are processive?
vkraynov at pop.service.ohio-state.edu
Tue Jun 3 21:43:41 EST 1997
In article <338DED79.53CE at usc.edu>, adleman at usc.edu wrote:
>We are interested in enzyme processivity.
>1. We know that polymerases are processive.
>Though in this regard, which DNA-polymerases incorporate the most
>nucleotides before falling off?
If the processivity = kcat/koff, then - most processive are the replicative
enzymes - T7 Pol, T4 Pol, E.coli Pol III, mammalian pol alpha. For T7 Pol
processivity is around 1500 nt/binding event.
Repair polymerases are significantly less processive (pol beta - 3-30
nt/binding, and is often considered a distributive enzyme).
>2. Are some restriction enzymes processive?
Meaning ? Restriction enzyme is supposed to make a SINGLE cut per binding
event, right ?
>3. Are some ligases processive?
Same with ligases
>4. Are there any other processive enzymes?
Any polymerase ? (Procesivity >/ 1 )
>adleman at cs.usc.edu
More information about the Methods