Why G418 and not neomycin?

Gregers Gram Gregers.Gram at inet.uni-c.dk
Tue Jun 3 08:24:56 EST 1997


Hello

As far as I´m infromed its it because of the purity. Neomycin is only about
80% pure and possibly contain toxic impurities.

 G418 is a chemically synthesized neomycin analoge and doesn´t contain
impurities, eventhough the specific activity is >100 %.

Gregers

:::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
: Gregers J. Gram, cand. scient.
: Lab. Infect. Dis. 144
: Hvidovre Hospital
: Denmark
: EMAIL: Gregers.Gram at inet.uni-c.dk
: WWW: http://inet.uni-c.dk/~mamocell/gg/
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
 Wolfgang Schechinger wrote in article <5m9dbm$5uq at mserv1.dl.ac.uk>...
>Hi experts!
>
>Why is G418 and not Neomycine itself
>used to selcet for transferred genes
>in eukaryotes
>(using neomycin phosphotransferase
>as marker gene) ?
>Is neomycine not toxic for eukaryotes?
>
>Wolfgang
>-----
>                                    
>Wolfgang Schechinger        
>University of Tuebingen
>email: wgschech at med.uni-tuebingen.de
>
>http://www.medizin.uni-tuebingen.de/~wgschech/research.htm
>
>public PGP key is avilable on request
>
> 





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