Homemade Chemiluminescent Protocols?

[Mark Dalton]

I tried this once it was much less sensitive than super signal from 
Pierce Chemicals.  By the way Pierce is 1/2 the price of ECL from 
Amersham, and is more sensitive.  I suggest you try the homemade stuff 
for yourself but make sure you do the controls the chemicals were very 
cheap around $20.  I found protocol in the Red Book.  It is listed in 
the other replies.

Good Luck 
Mark

Thomas Cameron wrote:
> 
> Company kits end up costing $3-5 per western blot which is 90% of the
> cost of the whole procedure.
> 
> People must have some good homemade HRP (and AlkPhos) Chemiluminescent
> Detection protocols or references?
> 
> Could you send me one or point me to it?  Thanks.
> 
> --
> Thomas Cameron