T/A cloning vector ??? Help???
zchai at COBRA.PATH.MONASH.EDU.AU
Wed Jun 4 20:29:18 EST 1997
>Does someone have a protocols for constructing the T/A cloning vector ???
>who can help me ????
Some one published a method a while (1-2yrs or more) ago to make T/A
vector. What you need to do is cutting your vector blunt ended, for example
using HincII, SmaI or EcoRV, adding ddTTP to the 3'end at both ends by TdT.
Use ddTTP instead of dTTP because you want only a single T at the end. I
have never go any chance to try this, but think the design is rational and
Hope this of help.
Dr. ZhongLin Chai
Department of Pathology and Immunology
Monash University Medical School
Commercial Rd, Prahran, VIC 3181, AUSTRALIA
Telephone: (61 3) 9276 2698 (lab)
(61 3) 9276 2696 (office)
(61 3) 9384 1305 (home)
0419 87 1940 (mobile)
+ 61 419 87 1940 (international mobile)
Fax: (61 3) 9529 6484
email: zchai at cobra.path.monash.edu.au
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