T/A cloning vector ??? Help???

[Dom Spinella]

Dong Liu writes:

> Hello all,
> 
> Does someone have a protocols for constructing the T/A cloning vector ???
> who can help me ????
> Thanks,
> 
> Dong Liu

See Borovkov and Rivkin, BioTechniques 22: 812-814 (1997). They describe
the construction of a cloning vector using oligos containing a pair of
XcmI sites to generate the 3' T overhangs. Cheers.  -- D.G. Spinella