Improving quantitative PCR

Edgar Valencia edgar at CIFN.UNAM.MX
Thu Jun 5 13:31:37 EST 1997

Hi netters:
One of my friends who is working with Toxoplasma wants to do some
quantitative PCR from infected tissue. Her problem is she's not having
enough sensitiveness, her PCR doesn't work when she has less than 250 pg
DNA. Is there something she could do to detect lower amounts of DNA?
Thanks in advance

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