Why G418 and not neomycin?

Wolfgang Schechinger wgschech at med.uni-tuebingen.de
Thu Jun 5 11:31:37 EST 1997


Hi. I would like to add something:

As far as I know now, Neomycine is against Eukaryotics only. It binds 
to their ribosomes and blocks their protein synthesis. Since 
eucaryotic ribos are different, Neo won't be able to block protein 
synthesis there. But G418 does (it was engineered for that).

The clue is that the NeoR Gene that's known from bacteria may be used 
for selection in eucaryotics, too. Saves disk ahem plasmid space.

Cheers to all, 

Wolfi

> Hello
> 
> As far as I=A6m infromed its it because of the purity. Neomycin is
> only about 80% pure and possibly contain toxic impurities.
> 
>  G418 is a chemically synthesized neomycin analoge and doesn=A6t
>  contain
> impurities, eventhough the specific activity is >100 %.
> 
> Gregers
> 
> ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: :
> Gregers J. Gram, cand. scient. : Lab. Infect. Dis. 144 : Hvidovre
> Hospital : Denmark : EMAIL: Gregers.Gram at inet.uni-c.dk : WWW:
> http://inet.uni-c.dk/~mamocell/gg/
> ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
>  Wolfgang Schechinger wrote in article
>  <5m9dbm$5uq at mserv1.dl.ac.uk>...
> >Hi experts!
> >
> >Why is G418 and not Neomycine itself
> >used to selcet for transferred genes
> >in eukaryotes
> >(using neomycin phosphotransferase
> >as marker gene) ?
> >Is neomycine not toxic for eukaryotes?
> >
> >Wolfgang
> >-----
> >                                    
> >Wolfgang Schechinger        
> >University of Tuebingen
> >email: wgschech at med.uni-tuebingen.de
> >
> >http://www.medizin.uni-tuebingen.de/~wgschech/research.htm
> >
> >public PGP key is avilable on request
> >
> > 
> 
> 
> 
> 
-----
                                     
Wolfgang Schechinger         
University of Tuebingen
email: wgschech at med.uni-tuebingen.de

http://www.medizin.uni-tuebingen.de/~wgschech/research.htm

public PGP key is avilable on request	




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