Single-copy eukaryotic Southern...

[mbthiam at NEXUS.MICROIMM.MCGILL.CA]

Hi!

If anyone could help me out it would be much appreciated!

I am looking for protocols that would allow me to do single-copy 
eukaryotic genomic Southerns, as I am looking for possible homologues 
to a human gene in other eukaryotic organisms (mouse, rat, 
chicken, Guinea pig, calf, ...). My problem lies in the fact that, using 
a 294 bp cDNA fragment which encodes the DNA-binding 
region (88 aa) of the much larger human protein (1093 aa), I have not
been able to get a signal when performing a Southern with my 
control (human epithelial cell genomic DNA).

Thanks for your time and attention!

Madani B. Thiam
Ph.D. student, Dept. of Microbiology and Immunology
McGill University
Montreal, CANADA
mbthiam at microimm.mcgill.ca