Electrocompetent cells

Mike O-Donehue odonohue at jouy.inra.fr
Fri Jun 6 07:16:11 EST 1997


Can anyone give me a protocol for the preparation of electrocompetent coli
which will give efficiencies greater than 10exp8 transformants / microgramme? 
Whatever the protocol , we always seem to be limited to 10exp6-10exp7, even 
when strains such as DH5a or XL1 blue are used.

Michael O'Donohue
odonohue at lille.inra.fr




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