Improving quantitative PCR

[Dr. Duncan Clark]

In article <Pine.SOL.3.95.970605123413.27996B-100000 at uxmal>, Edgar
Valencia <edgar at CIFN.UNAM.MX> writes
>
>Hi netters:
>One of my friends who is working with Toxoplasma wants to do some
>quantitative PCR from infected tissue. Her problem is she's not having
>enough sensitiveness, her PCR doesn't work when she has less than 250 pg
>of
>DNA. Is there something she could do to detect lower amounts of DNA?
>Thanks in advance
>Edgar
>

Nested PCR?

Duncan
-- 
The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
TEl/FAX 01252376288
http://www.dnamp.com
http://www.genesys.demon.co.uk