Dissolve Zinc Chloride in Cell Culture Medium

Frank O. Fackelmayer fof1 at chclu.chemie.uni-konstanz.de
Fri Jun 6 10:14:53 EST 1997


In article <01bc70f7$cfe11900$a5628bd0 at default>, "Suen Yick Keung"
<yksuen at netvigator.com> wrote:

> I am going to test the possible inhibitory effect of zinc ions on apoptosis
> of murine macrophage cell line.  It is well known that zinc can inhibit
> apoptosis in many cell types and I found there are many papers showing
> reduction of apoptosis of culture cells in the presence of zinc chloride. 
> At the beginning of my experiment, I faced the most fundamental but
> important problem:  the solubility of zinc chloride in neutral pH culture
> medium.  Obviously, the zinc chloride  cannot dissolve in the medium when
> the pH is near neutral (around pH 7.2).  No paper (as far as I have
> obtained) mentioned this (may be it is too simple).  And I was unable to
> find the solution from any chemistry books.
> 
> Do anyone know the answer ? Please Email to me.
> 
> Thanks !!
> 
> Y.K.Suen
> 
> Email :  yksuen at netvigator.com



Try zinc sulfate, it dissolves up to 1M (at least). 
Hope this helps,
Frank

-- 
Dr. Frank O. Fackelmayer
Division of Biology
University of Konstanz
D-78434 Konstanz
Germany



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