Dissolve Zinc Chloride in Cell Culture Medium
Frank O. Fackelmayer
fof1 at chclu.chemie.uni-konstanz.de
Fri Jun 6 10:14:53 EST 1997
In article <01bc70f7$cfe11900$a5628bd0 at default>, "Suen Yick Keung"
<yksuen at netvigator.com> wrote:
> I am going to test the possible inhibitory effect of zinc ions on apoptosis
> of murine macrophage cell line. It is well known that zinc can inhibit
> apoptosis in many cell types and I found there are many papers showing
> reduction of apoptosis of culture cells in the presence of zinc chloride.
> At the beginning of my experiment, I faced the most fundamental but
> important problem: the solubility of zinc chloride in neutral pH culture
> medium. Obviously, the zinc chloride cannot dissolve in the medium when
> the pH is near neutral (around pH 7.2). No paper (as far as I have
> obtained) mentioned this (may be it is too simple). And I was unable to
> find the solution from any chemistry books.
> Do anyone know the answer ? Please Email to me.
> Thanks !!
> Email : yksuen at netvigator.com
Try zinc sulfate, it dissolves up to 1M (at least).
Hope this helps,
Dr. Frank O. Fackelmayer
Division of Biology
University of Konstanz
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