mutant neo marker?

[Brett Beitzel]

Anyone out there have a construct with a mutant neomycin
phosphotransferase II as described in Yenofsky, et al  PNAS 87: 3435-3439
(1990)?
Basically, they found a E182D mutation that reduced the activity of the
enzyme quite dramatically, and I would like to use that mutant enzyme in
some of my constructs.

Any help would be greatly appreciated.

Thanks,
Brett Beitzel
bbeitzel at jeeves.ucsd.edu

Brett Beitzel
Infectious Disease Lab
Salk Institute
bbeitzel at jeeves.ucsd.edu