Need Fast & Cheap Plasmid Miniprep Kit
R.SKILTON at CGNET.COM
Fri Jun 6 12:10:43 EST 1997
If you prefer to use a kit, I have found that you can use a Wizard miniprep
kit using half of all the reagents to give essentially a half yield (but
still enough for doing many sequencing reactions or several digests). The
push/vacuum columns can be washed out and reused several times.
To: bionet.molbio.methds-reagnts mail newsgroup
Subject: Re: Need Fast & Cheap Plasmid Miniprep Kit
Date: Thursday, June 05, 1997 10:07AM
Emad Gharavi (Emad_G at MSN.COM) wrote:
: Dear All:
: Without inciting a war of the KITs, I'd like to ask if anyone knew of a
: but decent, commercial miniprep kit. We will routinely be doing
: and sequencing so a fast, reliable and high quality kit would be nice.
: used Qiagen's kits and would normally use them except they're a bit costly
: our budget. And I wouldn't want to be the one to tell our clinical
: that they'll have to do it the old fashioned way.
: All suggestions would be appreciated
: E Gharavi
: LSU Medical Center
: New Orleans, LA
: Emad_G at msn.com
I used the promega wizard kits and I found it VERY reliable and wasable to
produce excellent sequence.Its one of the cheaper kits as far as I know.
However I have found that simply doing the Maniatis alkaline lysis prep
equally good results....do you really have to use a kit?
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