how many nucleotides are required for restriction enzyme digestion of biotinylated DNA?

ISOAI Atsushi aisoai at agc.co.jp
Mon Jun 9 10:08:19 EST 1997


yshan at bioneer.kaist.ac.kr (Hahn Yoonsoo) wrote:

> I have to construct a linker which is biotinylated and will be cut by
>restriction enzyme later. How many nucleotides are required between
biotin
>and the restriction site? Does biotin inhibit the enzyme if it is very
close
>to the restriction site?
>
>  biotin-NNNNNNNGAATTCTTTTTTTTTTTTTTTT
>         ^^^^^^^
>         How many N's are needed?

Hi,
You should add "at least" two or more nucleotides (N's) at 5'-end
of restriction sites.
bye.

-- 
===================================================
Atsushi Isoai, Ph.D.    |   <aisoai at agc.co.jp>
Research Center, Asahi Glass Co.LTD., JAPAN
URL: http://www.yk.rim.or.jp/~aisoai/molbio-j.html
===================================================



More information about the Methods mailing list