how many nucleotides are required for restriction enzyme digestion of biotinylated DNA?
aisoai at agc.co.jp
Mon Jun 9 10:08:19 EST 1997
yshan at bioneer.kaist.ac.kr (Hahn Yoonsoo) wrote:
> I have to construct a linker which is biotinylated and will be cut by
>restriction enzyme later. How many nucleotides are required between
>and the restriction site? Does biotin inhibit the enzyme if it is very
>to the restriction site?
> How many N's are needed?
You should add "at least" two or more nucleotides (N's) at 5'-end
of restriction sites.
Atsushi Isoai, Ph.D. | <aisoai at agc.co.jp>
Research Center, Asahi Glass Co.LTD., JAPAN
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