urea in polyacrylamide gels?

Alexander N. Kukushkin kan at mmcd.cyt.ras.spb.ru
Mon Jun 9 10:05:06 EST 1997


>   In the preparation of a typical polyacrylamide gel, one adds urea,
>   ammonium persulfate, TEMED, and 10X TBE to the gel solution (we usually
>   use Long Ranger(ABI)).  What is the function of the urea?
>
>   Michael Heuer, undergraduate
>   University of Minnesota, Morris
>
Urea does supply with denaturated state of DNA in a gel.
Alex



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