Protein elution from SDS-PAGE

Kajiwara Hideyuki kajiwara at abr.affrc.go.jp
Mon Jun 9 20:02:04 EST 1997


akopian at bu.edu wrote:
> 
> Can somebody please send me a simple protocol for the elution of proteins
> from SDS-PAGE gels.  I've read about the electroelution equipment that
> are out their, but I would like to avoid it if possible...Is there a
> simple technique or apparatus I could set up for doing this?  Thanks in
> advance...Please respond to akopian at bu.edu.

Simplest way to elute protein from gel is the electrophoresis in the
dialysis tube on the horizontal electrophoresis apparatus.   You can
observe that CBB is out from gel (1 W, abou 60 min).  Then, start the
dialysis against a buffer after removing gel from the bag.



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