electrocompetent cell prep. (pLysS containg cell)

Pink Floyd joonho at bomun.kaist.ac.kr
Sun Jun 8 22:55:46 EST 1997


Hi!!

Do anyone know electro-competent cell (pLysS containg cell) prep.?

I tried to make electro-competent cell using conventional method.

However, after store -70 C, the cells are lysed.

XL1-Blue strain was made successfully using same method.

Do anyone have special protocol?

Thank you..



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