Recombinant Lac Repressor

[ijiwaru at wheel.dcn.davis.ca.us]

In article <33963576.8D7 at mdc-berlin.de>, cartier at mdc-berlin.de (Cartier) wrote:

> Hello everybody,
> 
> Does anyone know a Company selling purified recombinant lac repressor
> protein ?
> 
> Otherwise, is there any source of wild-type lacI repressor protein
> expression vector for production in E.Coli ? Tagged version of the
> protein for affinity purification would be great too.
> 
> Thanks in advance,
> 
> Regis Cartier
> Medical Biotechnology Center
> Institute of Human Virology
> University of Maryland at Baltimore, MD, USA.

Regis,

if I'm nont mistaken, pGEX-2T (and most of the pGEX vectors from
Pharmacia) carry a copy of lacIq which is not altered in the repressor
coding sequence.  you could try making oligos and PCRing out of that.

Lyle Najita
Plant Pathology
University of California - Davis