short sequencing

Lynn Hughes Lynn.Hughes at jcu.edu.au
Mon Jun 9 22:50:53 EST 1997


Hi all,

Just hoping for some suggestions with a sequencing problem we are 
having.  We are wanting to obtain the sequence of our DNA about 20bp 
from where the primer attaches.The closest we have achieved so far is 
about 40bp.  Has anyone got any suggestions on how to get closer to 
the primer attachment region.

Thanks for your help.

Lynn Hughes
Dept of Biochemistry and Molecular Science
James Cook University of North Queensland
Australia

email  lynn.hughes at jcu.edu.au



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