urea in polyacrylamide gels?

Brick Ayola bayola at b.imap.itd.umich.edu
Mon Jun 9 17:12:31 EST 1997

According to BioRad (Bulletin 1156) Urea has the effect of smaller pore
size gels presumably due to disruption of hydrogen bonds during

Luck & Grant Money,


On Sun, 8 Jun 1997, Michael L. Heuer wrote:

> In the preparation of a typical polyacrylamide gel, one adds urea,
> ammonium persulfate, TEMED, and 10X TBE to the gel solution (we usually
> use Long Ranger(ABI)).  What is the function of the urea?
> Michael Heuer, undergraduate
> University of Minnesota, Morris

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