urea in polyacrylamide gels?

[Brick Ayola]

According to BioRad (Bulletin 1156) Urea has the effect of smaller pore
size gels presumably due to disruption of hydrogen bonds during
polymerization.

Luck & Grant Money,

Brick


On Sun, 8 Jun 1997, Michael L. Heuer wrote:

> 
> In the preparation of a typical polyacrylamide gel, one adds urea,
> ammonium persulfate, TEMED, and 10X TBE to the gel solution (we usually
> use Long Ranger(ABI)).  What is the function of the urea?
> 
> Michael Heuer, undergraduate
> University of Minnesota, Morris
> 
> 
> 
>