Protein elution from SDS-PAGE

ISOAI Atsushi aisoai at agc.co.jp
Tue Jun 10 10:17:44 EST 1997


In article <339CA78C.5489 at abr.affrc.go.jp>, kajiwara at abr.affrc.go.jp
wrote:

>akopian at bu.edu wrote:
>> 
>> Can somebody please send me a simple protocol for the elution of
proteins
>> from SDS-PAGE gels.  I've read about the electroelution equipment that
>> are out their, but I would like to avoid it if possible...Is there a
>> simple technique or apparatus I could set up for doing this?  Thanks
in
>> advance...Please respond to akopian at bu.edu.
>
>Simplest way to elute protein from gel is the electrophoresis in the
>dialysis tube on the horizontal electrophoresis apparatus.   You can
>observe that CBB is out from gel (1 W, abou 60 min).  Then, start the
>dialysis against a buffer after removing gel from the bag.

Easier way is:
Only soak the gel containing the target protein in the suitable
buffer overnight. Agitation may be helpful (but not essential).

-- 
===================================================
Atsushi Isoai, Ph.D.    |   <aisoai at agc.co.jp>
Research Center, Asahi Glass Co.LTD., JAPAN
URL: http://www.yk.rim.or.jp/~aisoai/molbio-j.html
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