Protein elution from SDS-PAGE
aisoai at agc.co.jp
Tue Jun 10 10:17:44 EST 1997
In article <339CA78C.5489 at abr.affrc.go.jp>, kajiwara at abr.affrc.go.jp
>akopian at bu.edu wrote:
>> Can somebody please send me a simple protocol for the elution of
>> from SDS-PAGE gels. I've read about the electroelution equipment that
>> are out their, but I would like to avoid it if possible...Is there a
>> simple technique or apparatus I could set up for doing this? Thanks
>> advance...Please respond to akopian at bu.edu.
>Simplest way to elute protein from gel is the electrophoresis in the
>dialysis tube on the horizontal electrophoresis apparatus. You can
>observe that CBB is out from gel (1 W, abou 60 min). Then, start the
>dialysis against a buffer after removing gel from the bag.
Easier way is:
Only soak the gel containing the target protein in the suitable
buffer overnight. Agitation may be helpful (but not essential).
Atsushi Isoai, Ph.D. | <aisoai at agc.co.jp>
Research Center, Asahi Glass Co.LTD., JAPAN
More information about the Methods