Improving quantitative PCR
Wai Kwong Lee
wkl1j at clinmed.gla.ac.uk
Wed Jun 11 07:17:33 EST 1997
In article <Pine.SOL.3.95.970605123413.27996B-100000 at uxmal>,
edgar at CIFN.UNAM.MX says...
Here is a quick way of improving your yeild, Why don't you add w1 detergent to
your Pcr rxn and increase the No of cycles. You can get this from Gibco BRL
Cat No 12287-017. I usually add 1ul to a 20ul total rxn volume. I beleive
this incereses the lifetime of the polymerase. Good luck and email us back to
let us know how you got on.
best wishes Wai kwong Lee
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