PCR Machines and refridgeration

Deb Schuback schuback at helix.mgh.harvard.edu
Wed Jun 11 11:11:15 EST 1997

The only reason that I prefer a PCR machine to have refridgeration is that
I can also use it for ligations and the random RE that is at room temp or
below! I also agree though that keeping PCR reactions at 4 degrees when
they are finished is a bit ridiculous. I mean hey, they've spent about 20
minutes at 94 degrees. What could be in there?

On 10 Jun 1997, Alex Dobrovic wrote:

> I remain unconvinced that it is necessary to bring the samples to 4oC and
> maintain them at this temperature after the PCR has finished. There is no

Schuback at helix.mgh.harvard.edu
Lab Manager/Senior Tech
Massachusetts General Hospital
Molecular Neurogenetics

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