RNA precipitation with LiCl

Andreas Vogel vogela at uni-muenster.de
Thu Jun 12 10:35:54 EST 1997


Dear all,

i´m isolating RNA from bean seeds using various protocols which utilize a
precipitation in 3M LiCl as the final step. My pellet becomes very big and
white and could not be cleaned by several washing steps with 70% EtOH. Does
anybody know how to avoid this coprecipitation (of LiX?) or to clean the
RNA?
By the way, does anybody knows why Li makes a difference between RNA and DNA?

Thanks in advance for your help,
Andreas




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