Does Tris break down in electrophoresis?

Lou Cantolupo cantolupo at GENVEC.COM
Thu Jun 12 10:47:27 EST 1997

As far as I know it doesn't break down, but what happens is that if you
use your running buffer over and over again, salt from the samples will
leach into the buffer changing the conductivity.  I use my buffer about
5 times before changing it.

Lou Cantolupo
GenVec, Inc.
Dept. Molecular Virology
12111 Parklawn Drive
Rockville, MD  20852

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