Is ligation reaction volume only 10 ul?

Yeon Chul Kim e_yckim at cais.kaist.ac.kr
Fri Jun 13 01:03:33 EST 1997


Hi!!

In my knowledge, most people used 10 ul of ligation reaction volume.

Are there some problems using greater than 10 ul (for example 50 or 100
ul)?

Please give helpful information.

Thank you..
--
Yeon Chul Kim
BPERC, KAIST
373-1, Kusong-dong, Yusong-gu
Taejon 305-701, Korea
Tel) 82-42-869-3952
Fax) 82-42-869-8800
E-mail) e_yckim at cais.kaist.ac.kr
URL:  http://chiak.kaist.ac.kr/~yckim/



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