How to get active protein out of inclusion bodies?

Chris Kafer ckafer at iastate.edu
Thu Jun 12 00:00:42 EST 1997


My 55 kD Arabidopsis protein, expressed as a GST fusion, is nearly
100% in the post sonicate pellet.  I am looking for a method to purify
the protein with GST-Sepharose that will give me active protein (after
cleavage with Factor Xa).

Any protocols or thoughts on this subject will be most appreciated!

Thanks!


************************************************************************
Chris Kafer	www.public.iastate.edu/~ippm	       PGP Key available
		www.public.iastate.edu/~thorn
************************************************************************



More information about the Methods mailing list