Coomassie staining before electroblotting?

Chris Kafer ckafer at iastate.edu
Thu Jun 12 00:10:48 EST 1997


Can/should an SDS protein gel be stained with coomassie before
electroblotting onto nitrocellulose which will be followed by
detection with antibodies and protein A?  I thought not, but am not
sure why!

Thanks!


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Chris Kafer	www.public.iastate.edu/~ippm	       PGP Key available
		www.public.iastate.edu/~thorn
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