PCR from lambda plaques ??

Fredrik Kamme fkhero at biogen.wblab.lu.se
Fri Jun 13 06:31:19 EST 1997


Hi,
I do PCR on the plug eluates. Take 0.5µl out of 500µl SM buffer with the
plug. Denature the template at 95-100° before PCR, and run 35 cycles
(30µl tot vol). Has yet to fail me.

Greetings, Freddy



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