DNA elution from PAGE

James Zacka jzacka at worthington-biochem.com
Fri Jun 13 13:44:57 EST 1997


Worthington Biochenical, www.worthington-biochem.com, offers a rapid spin
column based kit for the recovery of DNA/RNA from gels with typical
recovery >80%.
In article <339E55BA.1B02 at sun-recomgen.univ-rennes1.fr>, Yvon CAVALOC
<cavaloc at sun-recomgen.univ-rennes1.fr> wrote:

> I did it very simply before:
> 1/ cut the band
> 2/ cut this band in small pieces (1-2 mm)
> 3/ put all these acrylamide fragments in an eppendorff tube and fill
> with TE (500 ul) and 250 mM NaCl
> 4/ leave it on a rocker over night at 4 degrees
> 5/ the day after, one chloroforme extraction, and add 2.5 vol of cold
> ethanol to precipitate
> 
> Recovery for a 500 bp in a 6% (never go under this concentration)
> Acrylamide gel: >75%
> I used this technique for fragments from 20 to 3000 bp and the results
> was really excellent for cloning
> Hope This will help
> 
> Yvon
> 
> Dr Yvon CAVALOC
> UPR41 CNRS "Recombinaisons Genetiques"
> Faculte de Medecine
> 2, Av du Pr Leon Bernard
> 35043 RENNES Cedex - France
> Tel +33 (0)2 99 33 62 47
> Fax +33 (0)2 99 33 62 00
> mail cavaloc at sun-recomgen.univ-rennes1.fr



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