Site Directed Mutagenesis Kits
Richard J. Dudley
rdudley at nrc.uab.edu
Mon Jun 16 13:31:26 EST 1997
Vernon Wayne Bauer wrote:
> I need to do some basic site-directed mutagenesis. Nothing special. It
> seems to me one of the commercial kits should be the easiest way, but I know
> nothing about any of them. Does anyone have any thoughts, good/bad experiences,
> recommendations, etc. they could pass on? Thanks for your help.
> Vernon Bauer
We use Promega's Altered Sites kit and always obtain good results. You
have to use the single stranded DNA protocol, though--us and several
other labs haven't had much success with the "new" method. This kit
requires youy to clone into pAlter, but I think they're coming out with
one that doesn't require additional subcloning.
We get between 50-90% mutagenesis about 98% of the time. We've made
maybe 50 mutants this year alone, and only one didn't mutate the first
try (second try worked, though). Very rarely fails us.
(no affiliation, just a happy customer).
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Richard J. Dudley (rdudley at nrc.uab.edu)
Department of Neurobiology
University of Alabama School of Medicine
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