Transfecting pMSG into COS-7 cells

[Kitty Bos]

Hi, I am wondering whether there is anyone who has used the mammalian 
inducible expression vector pMSG (Pharmacia). At Pharmacia they 
haven't been able to tell me how leaky the promotor is (MMTV LTR 
promotor). As I would like to use this vector in COS-7 cells (African 
green monkey kidney), I was wondering whether these cells are 
glucocorticoid (dexamethasone) responsive in order to induce 
expression. Any help is more than welcome. Thanks

Kitty Bos
Lion's Eye Institute
Perth, Australia
kbos at cyllene.uwa.edu.au