pCS2+ vector problems

Steven Sullivan sullivan at gwis2.circ.gwu.edu
Tue Jun 17 22:16:59 EST 1997



I'm having a hell of a hard time cloning into this vector and the related
pCS2+MT (myc tagged).  Anyone else had problems with these, and more
importantly, tips on getting inserts into them?  Two of the problems I'm
seeing are odd restriction patterns when cut with Xho and XbaI, and
self-religation even after extensive double-cutting.   






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