Difference between Nest-PCR and Two-Round PCR

ma ddsyr at NS.EAST.CN.NET
Tue Jun 17 08:09:34 EST 1997

Dear Netters:
It is known that when the first round PCR get little product to be seen in gel, you can 
use nest pcr. I just wonder what is the difference if we use same pairs of primers to do 
two round pcr? Thanks for seggestion.
Dr Q. H. Ma
Institute of Botany, Academia Sinica
Beijing 100093, China

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