A Peculiar In Tranformation
ddsyr at NS.EAST.CN.NET
Tue Jun 17 08:09:31 EST 1997
I found a peculiar thing in transformation of E coli. After I got the transformant
colony, then isolated plasmid ( I use alkali method), usually I got good plasmid yield,
say 2 ul can see clearly in gel. But when I isolated plasmid from same colony (I keep
colony in LB plate in 4 C), then the yield became low, say 5-6 ul to see clearly in gel.
Anyone have experience of the same? and know the reason below it?
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