A Peculiar In Tranformation

ma ddsyr at NS.EAST.CN.NET
Tue Jun 17 08:09:31 EST 1997

Dear Netters:
I found a peculiar thing in transformation of E coli. After I got the transformant 
colony, then isolated plasmid ( I use alkali method), usually I got good plasmid yield, 
say 2 ul can see clearly in gel. But when I isolated plasmid from same colony (I keep 
colony in LB plate in 4 C), then the yield became low, say 5-6 ul to see clearly in gel.
Anyone have experience of the same? and know the reason below it?

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