293-T cells

Guriq Basi gbasi at slip.net
Tue Jun 17 13:50:12 EST 1997


David L. Haviland, Ph.D. wrote:
> 
> At 16:45 6/6/97 -0500, eric anderson wrote:
> >does anyone know where 293-T cells can be obtained?  i think it's a 293
> >kidney cell line stably transfected with SV40 Large T-Antigen (i'm asking
> >for someone else).  it's not in the ATCC online catalog.
> 
> Eric:
> 
> I regret that it is in the catalog.  It is listed at 293 under human,
> kidney.
> CRL-1573 price code J.
> 
> BTW- does anyone have any electroporation conditions that work for this
> cell line?
> 
> Hope this helps,
> David
> 
> =============================
>  David L. Haviland, Ph.D.
>  Asst. Prof. Immunology
>  University of Texas - Houston, H.S.C.
>  Institute of Molecular Medicine
>  2121 W. Holcombe Blvd.
>  Houston, TX  77030
>  Internet:"dhavilan at imm2.imm.uth.tmc.edu"
>  Voice: 713.500.2413  FAX: 713.500.2424
>  ------------------------------------------------------
> " Sometimes you're the windsheild, sometimes you're the bug."
> =============================
david,

we transfect 293 cells to ~30% efficiency by electorporation at 280V/960
µFd in a biorad electroporator. cells are concentrated to 20 million/ml
in optimem (BRL), and 0.5ml get zapped with 10-20 µg DNA (circular
plasmid) .  alternatively, invtrogens pFx-2 works just as well, if not
better, and requires less DNA and cells.  good luck,

gbasi at slip.net



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