Difference between Nest-PCR and Two-Round PCR
zyu at UNIXG.UBC.CA
Tue Jun 17 11:57:55 EST 1997
If you use the same primer pair in the second round, you may get
quite a bit non-specific PCR products, which are amplified from "a few"
nons-specific products in the first round. With nest PCR, you'll not have
On 17 Jun 1997, ma wrote:
> Dear Netters:
> It is known that when the first round PCR get little product to be seen in gel, you can
> use nest pcr. I just wonder what is the difference if we use same pairs of primers to do
> two round pcr? Thanks for seggestion.
> Dr Q. H. Ma
> Institute of Botany, Academia Sinica
> Beijing 100093, China
More information about the Methods