Difference between Nest-PCR and Two-Round PCR

[Zhongtang Yu]

If you use the same primer pair in the second round, you may get
quite a bit non-specific PCR products, which are amplified from "a few"
nons-specific products in the first round.  With nest PCR, you'll not have
such problem.

Zhongtang Yu
UBC

On 17 Jun 1997, ma wrote:

> Dear Netters:
> It is known that when the first round PCR get little product to be seen in gel, you can 
> use nest pcr. I just wonder what is the difference if we use same pairs of primers to do 
> two round pcr? Thanks for seggestion.
> Dr Q. H. Ma
> Institute of Botany, Academia Sinica
> Beijing 100093, China
> 
> 
> 
>