RT-PCR standard

Permana, Paska ppermana at phx.niddk.nih.gov
Wed Jun 18 12:40:25 EST 1997


Dear Netters:

Can anyone suggest good standards for RT-PCR? Many people have used 
beta-actin and glyceraldehyde 3 phosphate dehydrogenase as standards, but 
there are pseudogenes of both in the genome (human, mouse, rat) that may 
complicate the interpretation of the RT-PCR.

Detailed info will be much appreciated.

Paska Permana
Email: ppermana at phx.niddk.nih.gov



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