Is ligation reaction volume only 10 ul?

Minsoo Yoon yoonm at fri.cri.nz
Thu Jun 19 17:39:25 EST 1997


I don't think the reaction volume in ligation is very critical, just as
PCR reaction volume isn't. Usually 10 ul ligation mix is enough amount for
subsequent transformation. I do ligation in 20 ul volume in special
circumstances, and it's just fine.

In article <01bc77fe$cd0533c0$467df88f at KAISTWF.kaist.ac.kr>, "Yeon Chul
Kim" <e_yckim at cais.kaist.ac.kr> wrote:

> Hi!!
> 
> In my knowledge, most people used 10 ul of ligation reaction volume.
> 
> Are there some problems using greater than 10 ul (for example 50 or 100
> ul)?
> 
> Please give helpful information.
> 
> Thank you..
> --
> Yeon Chul Kim
> BPERC, KAIST
> 373-1, Kusong-dong, Yusong-gu
> Taejon 305-701, Korea
> Tel) 82-42-869-3952
> Fax) 82-42-869-8800
> E-mail) e_yckim at cais.kaist.ac.kr
> URL:  http://chiak.kaist.ac.kr/~yckim/



More information about the Methods mailing list