Q on freezing cells (QnD)
Dr David Winterbourne
sghk100 at sghms.ac.uk
Thu Jun 19 10:22:38 EST 1997
Wolfgang Schechinger wrote:
> Hi TC experts!
> Has anyone yet tried to freeze cells in the (micro)plate by just
> exchanging the media for FCS/DMSO (90/10) and storing them ant -80 or
> in Nitrogen? Will the cells get alive again after thawing?
> I know that's quick and dirty, but is theer a better way to get more
> than 50 clones (3T3 cells) into the fridge for four weeks?
Yes. It works perfectly well storing the plate (wrapped in cling film)
in a -80C freezer.
Dr. David Winterbourne
Department of Surgery
St George's Hospital Medical School, London SW17 0RE, England
Tel: 0181-725-5581 Fax: 0181-725-3594
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