blunt-end ligation with PCR product?

Colin Rasmussen crasmussen at anat.med.ualberta.ca
Thu Jun 19 14:33:42 EST 1997


Minh Luu wrote:
> 
> Help,
> 
> I'm trying to clone some PCR products, from pfu polymerase which
> generates blunt-ended products, into a plasmid cut with Ecl136II.  I've
> tried phosphatase treatment, adding PEG into my ligation, all without
> any success because all I see in my minipreps are false-positives
> (deletions mostly).  I'm thinking about getting Statagene's pCR-Script
> kit.  Can anyone offer some insight?  Thanks

Get the kit.  It has worked without fail for me.

Colin



More information about the Methods mailing list