blunt-end ligation with PCR product?
crasmussen at anat.med.ualberta.ca
Thu Jun 19 14:33:42 EST 1997
Minh Luu wrote:
> I'm trying to clone some PCR products, from pfu polymerase which
> generates blunt-ended products, into a plasmid cut with Ecl136II. I've
> tried phosphatase treatment, adding PEG into my ligation, all without
> any success because all I see in my minipreps are false-positives
> (deletions mostly). I'm thinking about getting Statagene's pCR-Script
> kit. Can anyone offer some insight? Thanks
Get the kit. It has worked without fail for me.
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