Looking for dam-/dcm- bact. strain

Steve Palmieri rocco at mail.utexas.edu
Mon Jun 23 14:59:25 EST 1997


I'm in dire need of a dcm-/dam- bacterial strain for cloning purposes.  I
have a construct with an ApaI site that is undergoing methylation(there is
a DCM methylation site via sequence analysis).  I've tried EB2007 but I
think our glycerol stock may be bad because they won't transform.  If
anyone has a methylation defective bacterial strain that they had success
with it will be much appreciated.  Thanks.

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