blunt-end ligation with PCR product?

Mike Perry mike.perry at bris.ac.uk
Mon Jun 23 10:46:29 EST 1997


Richard P Grant wrote:
> 
> Hi,
> 
> I know this may seem obvious, but sometimes that's what's missing.  With a
> phosphatased vector, have you got kinased PCR product?  I find it best to
> kinase the primers before the PCR.
> 
Don't joke - I did exactly that!!!!!!!ie forgot all about kinasing the
PCR product while trying to clone a blunt end peice!!

-- 
*************************************************************
Dr Andrew Doherty		email -  a.doherty at bris.ac.uk
Dept. Anatomy			Tel (0117)9287421
School of Medical Sciences	Fax (0117)9287402
University of Bristol
University Walk
Bristol UK
BS8 1TD
*************************************************************



More information about the Methods mailing list