blunt-end ligation with PCR product?

Mike Perry mike.perry at
Mon Jun 23 10:46:29 EST 1997

Richard P Grant wrote:
> Hi,
> I know this may seem obvious, but sometimes that's what's missing.  With a
> phosphatased vector, have you got kinased PCR product?  I find it best to
> kinase the primers before the PCR.
Don't joke - I did exactly that!!!!!!!ie forgot all about kinasing the
PCR product while trying to clone a blunt end peice!!

Dr Andrew Doherty		email -  a.doherty at
Dept. Anatomy			Tel (0117)9287421
School of Medical Sciences	Fax (0117)9287402
University of Bristol
University Walk
Bristol UK

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