Wanted:M13/T7 and/or JM109(DE3)

Peter Wang plw at mrc-lmb.cam.ac.uk
Mon Jun 23 10:46:55 EST 1997


Thon de Boer wrote:
> I'm looking for the M13 phage which contains the T7 RNA polymerase gene, so
>...
> (The M13 T7 gene is not under control of a lac promotor is it ?).

Such a phage is available from Invitrogen, I believe, but the it is under control of the 
lac promoter.

> I'm also looking for JM109(DE3) which is the normal JM109 with the DE3
> phage, which contains the T7 RNA Polymerase gene under control of a lac
> promotor.

JM109(DE3) is available from Promega.

---------------------------------------------------------
Peter Wang, M.D., Ph.D.
MRC Centre for Protein Engineering,
Hills Road, Cambridge, CB2 2QH, England

Tel (01223) 402104  (international calls +44-1223-402104)
Fax (01223) 402140  (     "          "   +44-1223-402140)
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