HeLa transient transfection

Fritz Grunert grunert at ruf.uni-freiburg.de
Tue Jun 24 02:53:30 EST 1997


Hi,
we try to transfect HeLa cells transiently with pRC/CMV or pBHE vectors
and CD66 inserts but have very poor expression (transfection with
lipofectamin (Gibco) 2µg DNA on 80% confluent HeLa cells, 6 well plate)
3-5% positive cells after 48h compared with CHO-cells. Has anyone an
idea or good protocol for efficient transfection of HeLa?

Fritz Grunert

Institute for Immunobiology,
University of Freiburg, Germany




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